Arabidopsis Thaliana Leaf Protoplast Preparation and Transformation Kit

Arabidopsis Thaliana Leaf Protoplast Preparation and Transformation Kit

The Arabidopsis leaf protoplast preparation and transformation kit integrates enzymatic digestion and PEG-mediated transformation. By optimizing the enzymatic digestion system, buffer osmotic pressure, and transformation conditions, it can prepare highly active protoplasts from leaves within 4-6 hours and achieve a transfection efficiency of 60%-80%. The kit includes enzymatic digestion solution, osmotic pressure balancing solution, PEG transformation reagent, and culture system. A 5 ml system can process approximately 0.1 g of leaves, yielding 500,000 to 700,000 protoplasts, meeting the high-throughput transfection needs of multi-well plates. The product can be stably stored at -20°C for one year and transported at room temperature. It is widely used for studies on transient gene expression, protein interaction, and CRISPR editing.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.