Minimal SD BaseEnglish nameMinimal SD BaseChinese nameMinimal SD Base培养基Specification267g/10×267gSample storageRTRansportation guideRoom temperature transportationMinimal SD Base is composed of ammonium sulfate, trace elements, and glucose mixed in proportion, and it is considered a minimal medium. It can provide the basic substances needed for the survival of wild-type yeast, but it does not contain the amino acids required by mutant strains. This medium needs to be used in conjunction with

GUS Staining KitEnglish nameGUS Staining KitChinese nameGUS染色试剂盒Specification50ml/100ml/10×50 mlSample storageFollow instructionsRansportation guideIce pack transportationThe GUS (β-glucuronidase, β-D-glucuronidase) gene is currently a commonly used reporter gene. Its expression product, β-glucuronidase, is a hydrolase that can catalyze the hydrolysis of many β-glucuronide compounds. It can decompose 5-bromo-4-chloro-3-indolyl-β-glucuronide (X-Gluc) into a blue substance. The detectio

X-a-gal (20mg/mlsterile, imported)English nameX-a-gal (20mg/mlsterile, imported)Chinese nameX-a-gal（20mg/ml无菌,进口）Specification1ml/5×1ml/10×1mlSample storage-20℃Avoid lightRansportation guideIce pack transportationX-α-Gal is a chromogenic substrate for yeast galactosidase (MEL1), used for directly detecting yeast two-hybrid interactions of the GAL4 system on agar plates. Positive blue clones can be quickly and easily identified through blue-white color screening, without the time-consu

SD/-Leu/-TrpBrothEnglish nameSD/-Leu/-TrpBrothChinese nameSD/-Leu/-Trp培养基Specification5L/5×0.5L/10×0.5LSample storageRTRansportation guideRoom temperature transportationYeast SD medium (Synthetic Dextrose Minimal Medium/ SD Minimal Medium/ SD-Dropout Medium), also known as yeast basic medium or Saccharomyces cerevisiae nutrient deficiency screening medium, is widely used in yeast interaction and yeast ion tolerance, functional complementarity, and the identification, screening and culture t

MSMedium（without Sucrose Agar）English nameMS Medium（without Sucrose Agar）Chinese nameMS培养基（不含蔗糖和琼脂）Specification50L/5×50L/10×50LSample storageRTRansportation guideRoom temperature transportationMS Medium (excluding sucrose and agar) is a plant tissue culture basic medium improved from the classic Murashige and Skoog formula. It is characterized by a high concentration of inorganic salts and is widely suitable for research and micropropagation production applications.1. This pro

Malondialdehyde (MDA) Detection KitEnglish nameMalondialdehyde (MDA) Detection KitChinese name过氧化氢（H2O2）检测试剂盒Specification100T/96SDetection methodmicromethodSample storage4℃（6 months）Ransportation guideIce pack shippingH2O2is the most common reactive oxygen species in organisms, mainly catalyzed and produced by SOD, XOID, etc., and catalyzed and degraded by CAT, POD, etc. H2O2is not only one of the important reactive oxygen species, but also the hub for the mutual transformation of

100×Modified Hoagland Nitrogen-Deficient Nutrient Solution(4Kinds of Stock Solutions)English name100×Modified Hoagland Nitrogen-Deficient Nutrient Solution (4Kinds of Stock Solutions)Chinese name100×改良霍格兰缺氮营养液（4种母液）Specification500mLSample storage4℃Ransportation guideIce pack transportation100 times improved Hogland nitrogen deficiency nutrient solution (4 mother liquors), is a high concentration nitrogen deficiency specific nutrient solution, with the core function of providi

SC/-Leu/-Trp BrothEnglish nameSC/-Leu/-Trp BrothChinese nameSC/-Leu/-Trp培养基Specification5L/10×5LSample storageRTRansportation guideRoom temperature transportationSC Medium is a yeast basal medium that does not contain a carbon source and lacks a glucose carbon source compared to yeast SD medium. Experimenters can add glucose, galactose, raffinose, fructose, sucrose, etc. as the carbon source of yeast according to the needs of the experiment, and use it to prepare yeast SD medium, SG medium,

YNB (without Amino Acids)English nameYNB (without Amino Acids)Chinese name酵母氮源基础(不含氨基酸)Specification100g/500gSample storageRTRansportation guideRoom temperature transportationYeast Nitrogen Base (YNB) is a basic nitrogen source for yeast, composed of a blend of nitrogen sources, trace elements, vitamins, and inorganic salts. It mainly provides a non-amino nitrogen source and growth factors for the cultivation of yeast (including Saccharomyces cerevisiae and Pichia pastoris). It is pri

Modified Hoagland Nitrogen-Deficient Nutrient Solution (Dry Powder + Concentrated Solution)English nameModified Hoagland Nitrogen-Deficient Nutrient Solution (Dry Powder + Concentrated Solution)Chinese name改良霍格兰缺氮营养液（干粉+浓缩液）Specification500LSample storage4℃Ransportation guideIce pack transportationThe core function of the improved Hoagland nitrogen-deficient nutrient solution (powder/concentrate) is to provide a precise nutritional environment for research on plant nitrogen def

6-BA Storage Solution(1mg/ml)English name6-BA Storage Solution(1mg/ml)Chinese name6 - 苄氨基嘌呤储存液（1mg/ml）Specification100mlSample storage-20℃Ransportation guideIce pack transportation6-BA Storage Solution (1mg/ml), also known as 6-Benzylaminopurine (6-BA) stock solution, is a commonly used cytokinin plant growth regulator stock solution in plant tissue culture. The key is to dissolve 6-BA powder to a concentration of 1mg/ml to prepare a stable stock solution for convenient dilution and u

Service IntroductionThe CRISPR/Cas9/gRNA (CRISPR-Cas RNA-guided nuclease) gene knockout technology is another major breakthrough following the TALEN gene knockout technology. Compared with TALEN and ZNF technologies, the CRISPR/Cas9 system is easier to construct and use. The specificity of gene knockout is determined by gRNA rather than protein, thereby avoiding the complex plasmid assembly process and even making it possible to knock out multiple genes simultaneously. Moreover, experiments have

Service IntroductionThe yeast one-hybrid technique is a tool developed based on yeast two-hybrid systems to study nucleic acid-protein interactions. It is widely used to investigate the regulation of gene expression in eukaryotic cells, such as identifying DNA binding sites, discovering potential binding protein genes, and analyzing information about DNA-binding domains.pHis2 system yeast one-hybrid:The pHis2 yeast one-hybrid system uses two plasmids, pHis2 and pGADT7-Rec2. The selectable marker

Service IntroductionThe yeast two-hybrid system is a research method for identifying and detecting protein-protein interactions. Due to its high sensitivity, powerful functionality, and wide applicability, it has now been applied in multiple research fields.Nuclear system yeast two-hybrid:The yeast two-hybrid system was originally established by Fields and others while studying the properties of the yeast transcription factor GAL4. With continuous improvements over time, it has developed into a

Service IntroductionThe yeast three-hybrid system was improved from the yeast two-hybrid system by introducing a plasmid pBridge that could simultaneously insert two exogenous proteins The bait protein was inserted into the MCS I of this plasmid, and the N terminus was still fused to the DNA-binding domain BD of the GAL4 system An exogenous protein regulated by the PMET25 promoter was inserted into the MCS II. The PMET25 promoter has the property of being able to start only whenionine is deficie

Service introductionCo-immunoprecipitation (Co-IP) is a classic method for studying protein-protein interactions based on the specific immune reaction between antibodies and antigens. Its basic principle involves adding antigen-specific antibodies to cell lysates. The antibodies, antigens, and proteins interacting with the antigens form immune complexes through an immunoprecipitation reaction. After purification, elution, and collection of the immune complexes, SDS-PAGE electrophoresis, Western

Service IntroductionThe fluorescent protein is split at specific sites to form two non-fluorescent polypeptides, the N-terminal and C-terminal fragments, referred to as the N-fragment and C-fragment. When these two fragments are co-expressed in cells or mixed in vitro, they cannot spontaneously assemble into a complete fluorescent protein and do not produce fluorescence when excited by the fluorescent protein's excitation light. However, when these two fragments of the fluorescent protein ar

Service IntroductionLuciferase refers to a group of enzymes in organisms that catalyze the oxidation of luciferin or fatty aldehydes to produce light, found in naturally luminescent organisms. Currently, the luciferase gene derived from the North American firefly (Photinus pyralis) is the most widely used. By utilizing the property of luciferase to undergo a chemiluminescent reaction with its substrate, the regulatory elements of the gene of interest can be cloned upstream or downstream of the f

Service IntroductionDetecting whether a protein's signal peptide has a secretory function and confirming whether a protein is a secreted protein is of central significance in both basic research and biotechnological applications. It directly affects whether a protein can be properly transported and perform its intended function. It not only addresses the basic biological question of 'where a protein works,' but also plays a key role in modern biotechnology in analyzing the pathogenic

Service IntroductionTobacco leaf subcellular colocalization analysis technology is a novel method for intuitively and rapidly determining the localization and interactions of target proteins within tobacco leaf cells. This technology cleverly fuses two different fluorescent protein molecules, green fluorescent protein (GFP) and red fluorescent protein (RFP), separately with the target proteins for expression. If the two fluorescent signals overlap to produce colocalization fluorescence, it indic

Service IntroductionThe yeast sugar transporter detection experiment refers to using yeast sugar-deficient mutants to test whether a protein expressed in yeast can transport the relevant sugars into yeast cells for utilization.Yeast Hexose Transport Activity DetectionSpecifically designed for studying yeast hexose (such as glucose and fructose) transporter protein functions, it is based on the principles of heterologous complementation and growth phenotype verification in yeast. This includes a