Sugar transport function test

Service Introduction

The yeast sugar transporter detection experiment refers to using yeast sugar-deficient mutants to test whether a protein expressed in yeast can transport the relevant sugars into yeast cells for utilization.

Yeast Hexose Transport Activity Detection

Specifically designed for studying yeast hexose (such as glucose and fructose) transporter protein functions, it is based on the principles of heterologous complementation and growth phenotype verification in yeast. This includes a core hexose transport-deficient Saccharomyces cerevisiae strain (such as EBY.VW4000), the pDR196 high-efficiency expression vector, and supporting culture medium reagents. The deficient strain, lacking all endogenous hexose transport proteins, cannot absorb hexose by itself and can only grow on maltose-containing media. After introducing the target transporter gene into the strain, an active protein can mediate hexose uptake, allowing the strain to grow normally on a medium with hexose as the sole carbon source. This specifically excludes interference from non-hexoses and is suitable for verifying hexose transporter protein functions, screening metabolic genes, and studying transport mechanisms.

Yeast Sucrose Transport Activity Detection

Specifically designed for studying yeast sucrose metabolism and transporter protein functions, it is based on the principle of heterologous expression in yeast. This includes a sucrose transport-deficient yeast strain (SUSY7/ura3), expression vectors, and supporting selective media reagents. The yeast mutant (SUSY7/ura3) cannot secrete invertase but can express a gene encoding a plant-derived cytosolic sucrose synthase, enabling yeast to metabolize sucrose intracellularly. This mutant strain, together with cDNA plasmids constructed on the yeast expression vector, is transformed, and the transformants are selected based on their growth ability on media with sucrose as the sole carbon source. This method intuitively reflects sucrose transport capability, excluding interference from other sugars, and is suitable for transporter protein function verification, sucrose metabolism gene screening, and regulatory mechanism studies.

Service Advantages

▶Standardization of mutant strains: Use internationally recognized strains to ensure experimental reproducibility, meeting the requirements of journals such as Nature and Plant Cell.

▶Substrate specificity verification: Use monosaccharide/disaccharide gradient media to rule out cross-reactions.

▶Data traceability: Provide original growth images, quantitative curves, and statistical analysis reports.

▶Custom service capabilities: Detection conditions can be optimized according to customer needs to suit the functional identification of special proteins.

Service Process

Yeast hexose transport activity assay

Yeast sucrose transport activity assay

Article Case

(1) Yeast hexose transport activity assay

Plasma membrane-localized SlSWEET7a and SlSWEET14 regulate sugar transport and storage in tomato fruits

academic journal：Horticulture Research

Analysis of the transport activity of SlSWEET7a and SlSWEET14 in yeast cells. a Growth of the yeast mutant strain EBY.VW4000 expressing different genes in SD (-Ura) media supplemented with different carbon sources-2% maltose, 2% fructose, or 2% glucose; yeast mutant strains transformed with the pDR195 empty vector were used as negative controls.

(2) Yeast sucrose transport activity assay

MdSWEET23, a sucrose transporter from apple (Malus × domestica Borkh.), influences sugar metabolism and enhances

cold tolerance in tomato

期刊：Frontiers in Plant Science

Yeast cells SUSY7/ura with pDR196-MdSWEET23 or pDR196 vector (as a negative control) were grown on SD/-Ura solid medium containing 2% (V/W) glucose, or 2% (V/W) sucrose as sole carbon source.

Applicable Fields

▶Plant Science: Crop stress resistance (salinity/alkalinity/drought), photosynthate allocation, seed development, functional validation of SWEET/MST family proteins.

▶Microbiology: Identification of pathogen virulence factors, modification of industrial yeast strains, study of adaptation mechanisms in extremophiles.

▶Synthetic Biology: Construction of standardized transport elements, functional validation after gene editing.