2×CPW Buffer (Sugar-Free)

2×CPW Buffer (Sugar-Free)

This product is a core basic reagent for the preparation of plant protoplasts. It is a 2× concentrated sterile basal salt solution, free of osmotic stabilizers such as mannitol and sucrose. The key components are potassium dihydrogen phosphate, potassium nitrate, calcium chloride, etc., and the pH is precisely adjusted to 5.8, which can stabilize the ionic environment of protoplasts and maintain cell membrane integrity. Users can independently add 0.3–0.7 mol/L mannitol or sucrose depending on the plant type, making it suitable for various plants including woody, herbaceous, and gramineous species. It can be stored at 4°C for 3 months or frozen at -20°C for 12 months. After sterilization with a 0.22 μm filter, it can be directly used for enzyme digestion system construction, protoplast purification, and PEG-mediated transformation. It is available in 25 mL, 100 mL, and 500 mL packaging. When used, the solution can be diluted 1:1 and is suitable for the preparation of leaf protoplasts from wheat, corn, pepper, tea tree, and other plants. It offers high cost-performance and is easy to operate.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.