IPTG Dioxane Free

IPTG Dioxane Free

IPTG is an inducer of β-galactosidase activity. In blue-white screening, IPTG acts as a gratuitous inducer to trigger protein expression in bacteria, while X-gal serves as a chromogenic substrate. The principle is that IPTG induces the Lac operon DNA segment of the vector to synthesize the amino-terminal fragment of β-galactosidase, which can complement the defective β-galactosidase encoded by the host cell (α-complementation). Bacteria exhibiting α-complementation, when plated on media containing the chromogenic substrate X-gal, form blue colonies. However, if foreign DNA is inserted into the multiple cloning site of the plasmid, it can disrupt α-complementation, and white colonies will grow on the medium. IPTG is also a commonly used inducer for recombinant protein expression in genetic engineering. 

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.