5-Fluoroorotic Acid(5-FOA)

5-Fluoroorotic Acid(5-FOA)

5-Fluoroorctic acid (5-FOA) is a commonly used molecular genetic screening method for yeast negative selection. 5-FOA is employed in yeast plasmid clearance, plasmid shuttle, positional exchange, and double-hybrid screening. 5-FOA itself is non-toxic to yeast cells, but the functional URA3 gene encodes and expresses orotic acid nucleoside-5'-monophosphate decarboxylase (OMP decarboxylase), which converts it into toxic forms (e.g., 5-fluorouracil), thereby inhibiting yeast cell growth. Consequently, in 5-FOA media supplemented with uracil, yeast strains carrying the URA3 mutant gene can grow normally. 5-FOA is primarily used for the identification and screening of Saccharomyces cerevisiae (URA3), although it has also been applied to other strains, including Schizosaccharomyces pombe (URA4 and URA5), Candida albicans (URA3), and Escherichia coli (pyrF). The use of 5-FOA in yeast double-hybrid systems enables the construction of activation domain hybridization libraries for the identification of protein-protein interactions.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.