Minimum nutrient V-B medium (bottom layer)

Minimum nutrient V-B medium (bottom layer)

Minimum nutrient V-B medium (bottom layer) is a solid medium specially used for bacterial conjugation test. Its core feature is that the nutrients are strictly limited, and it only contains the minimum salts and carbon sources (such as citrate) needed to maintain the basic survival of bacteria, but does not contain rich nitrogen sources (such as amino acids and nucleotides). This "nutritional deficiency" design is the key: it only allows the donor bacteria with specific nutritional markers (such as the ability to synthesize amino acids) to grow, and it is necessary to join with the recipient bacteria and obtain their complementary genes before forming colonies on the "upper" selection plate without this nutrition. Therefore, the bottom of the culture medium, as the basic platform for experiments, provides the necessary conditions for accurately screening and verifying the transfer of genetic materials (such as plasmids) between bacteria by creating specific nutritional pressure.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.