Amplification of cDNA library

Service Introduction

Expansion of the prokaryotic library refers to spreading the yeast hybrid prokaryotic culture onto 200 corresponding 150mm resistance plates, incubating overnight at 37°C, collecting all the colonies, extracting a large amount of plasmid, and performing identification before delivery.

Service Advantages

▶Highly experienced: With extensive experience in yeast hybrid library construction, our experienced team can help you quickly expand prokaryotic libraries;

▶Quality Assurance: By using the flat plate method, we ensure that each clone grows independently, avoiding competitive inhibition between genes and greatly reducing the chances of gene loss, thereby ensuring service quality.

Service Process

Article Case

Applicable Fields

▶Yeast Two-Hybrid Experiment: Provide sufficient library plasmids for target protein interaction screening, ensuring screening coverage and effectiveness.

▶Functional Gene Mining: Provide amplified libraries to support large-scale screening and validation of genes with unknown functions.

▶Library Resource Preservation: Achieve standardized amplification and long-term storage of yeast libraries to meet the needs of multiple subsequent experiments.

▶Drug Target Screening: Provide stable library materials for drug target screening platforms, aiding in the discovery of potential targets.