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Service Introduction Genome extraction and identification refers to extracting the genome from Pichia pastoris, Saccharomyces cerevisiae, plants, or other materials, designing specific primers, and detecting stably transformed positive clones through PCR. Service Advantages ▶Rich experience: We have extensive experience in yeast transformation, plant transformation, and nucleic acid extraction and identification. With over ten thousand targeted mutations performed annually, our experienced team can help you quickly complete genome extraction and identification; ▶Quality Assurance: Ensure that all gene extractions and identifications are authentic to maintain service quality. Service Process
Article Case
Applicable Fields ▶Verification of Yeast Engineered Strains: Applicable to engineered yeasts such as Pichia pastoris and Saccharomyces cerevisiae. By extracting the yeast genome and performing PCR detection, it verifies whether the exogenous target gene (e.g., recombinant protein expression gene) is stably integrated into the yeast genome, ensuring the successful construction of engineered strains and supporting subsequent research in fermentation production, protein expression, and more. ▶Plant Transgenic Research: Applicable to transgenic crops (such as insect-resistant cotton and disease-resistant maize) or model plants (Arabidopsis, tobacco). By extracting the genome from plant tissues (leaves, callus), it detects the integration of exogenous genes (stress-resistance genes, reporter genes), screens for stably inherited transgenic positive plants, and supports plant breeding and gene function studies. ▶Microbial Genetic Engineering Applications: Covers bacteria (such as E. coli, Agrobacterium) and fungi (such as Aspergillus, Penicillium). After extracting the microbial genome, PCR is used to identify whether the exogenous gene has been successfully transformed and stably maintained. This is used for screening engineered strains (such as degradative or enzyme-producing strains), promoting research in microbial fermentation, environmental remediation, and related fields. |
