Gene synthesis

Service Introduction

Gene synthesis refers to the technology of artificially synthesizing double-stranded DNA molecules in vitro, with a length range of 50 bp to 12 kb. Gene synthesis is a method of creating genes using artificial techniques and is one of the means to obtain genes. Compared to obtaining genes from existing organisms, gene synthesis does not require a template, and thus is not limited by the source of the gene. Through gene synthesis, genes that do not exist in nature can be obtained, opening up a completely new direction in the field of biomedicine. With continuous technological advancements, gene synthesis is playing an increasingly significant role in life sciences, and its applications in areas such as new energy, new materials, artificial life, nucleic acid vaccines, and biomedicine have begun to emerge. Wuhan Puint Bio can synthesize any DNA sequence you are interested in, providing accurate, fast, and high-quality whole gene synthesis services.

Service Advantages

▶The independently developed nucleases with ultra-long recognition sequences can accurately cut double-stranded DNA, and the cleavage sites can be customized as needed. They can be used for the assembly of large gene fragments, adding another valuable tool to synthetic biology;

▶The independently developed ultra-low temperature multi-segment recombinases can perform reactions and transformations on ice, truly achieving a "One Step" process;

▶The new technology process is more stable, has a shorter cycle, and is more cost-effective.

Service Process

Article Case

Applicable Fields

▶Industrial Enzyme Engineering: Synthesizing optimized industrial enzyme genes (such as highly active cellulase or thermostable protease genes), adapting them to different expression systems (E. coli, yeast), to improve enzyme production efficiency;

▶Agricultural Biotechnology Breeding: Designing genes for stress resistance or high-quality traits (such as insect-resistant Bt genes or genes related to high-oil crops), constructing plant expression vectors, and cultivating genetically modified crops;

▶Basic Research: Synthesizing genes of unknown function or mutant genes (for functional verification), constructing artificial promoters/regulatory elements, or synthesizing special DNA structures (such as hairpin structures or repeat sequences) to study molecular mechanisms;

▶Molecular Diagnostics: Synthesizing standard genes for nucleic acid testing (such as viral target gene fragments) and diagnostic probe genes to ensure the accuracy and standardization of testing reagents.