Yeast Transcription Activity Detection Kit

Yeast Transcription Activity Detection Kit

Herpes simplex virus protein 16 (VP16) is a transcriptional activator that can activate the expression of downstream genes. By fusing the VP16 transcriptional activation domain with GAL4BD and expressing it, the pGBKT7-VP16 (BD-VP16) vector was constructed and transformed into Y2HGold. The transcriptional activation function of VP16 will activate the expression of yeast reporter genes HIS3 and ADE2, allowing normal growth on histidine- and adenine-deficient media. Transcription factor X is fused separately with BD and BD-VP16 for expression. If transcription factor X has a transcriptional activation effect on downstream genes, it will activate their expression. Compared with the control strain containing BD, the strain containing BD-X can grow on deficient media. If transcription factor X has a transcriptional repression effect on downstream genes, it will partially inhibit the activation of downstream genes by VP16. Compared with the control strain containing BD-VP16, the strain containing BD-VP16-X will inhibit the activation of reporter genes downstream, thereby suppressing growth on deficient media. 

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.