NMY51 DUALhunter System Yeast Two-Hybrid interaction proving Kit

NMY51 DUALhunter System Yeast Two-Hybrid interaction proving Kit

The DUALhunter system is based on the DUALmembrane system, with appropriate modifications, i.e., by fixing cytoplas proteins (or nuclear proteins) to the cell membrane through a small membrane protein (yeast ER protein Ost4), which facilitates detection (see figure). First, the-terminus of the bait protein (Bait-Cub-LexA-VP16) is fused with a small membrane anchor protein (Ost4) to a fusion protein (Ost4-Bait-Cub-LexA-VP16); next, the target protein is fused to the N-terminus of ubiquitin NubG portion; if bait and prey interact, the two parts of ubiquitin, NubG and Cub, are brought close together and separated ubiquit is reconstituted, recognized by UBPs, leading to the dissociation of LexA-VP16, which enters the nucleus and thus activates the transcription of the reporter. 

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.