Y2HGold-GAL4 Yeast Two-Hybrid interaction proving kit

Y2HGold-GAL4 Yeast Two-Hybrid interaction proving kit

Basic Principle of Yeast Two-Hybrid: Many site-specific transcriptional activators in eukaryotes typically have two separable structural domains: a DNA-binding domain (BD) and a transcriptional activation domain (AD). Each of these domains has its own function and does not interfere with the other. However, a complete activator that can specifically activate gene expression must contain both domains simultaneously; otherwise, it cannot perform its activating function. When the BD of an activator from one source combines with the AD from another, it specifically activates the gene expression bound by BD. Based on this principle, two proteins of interest can be fused separately to these two domains and co-expressed in the same yeast cell. If the two proteins interact, they will act as a bridge to bring AD and BD together, forming a complete transcriptional activator and activating the corresponding reporter gene expression. By measuring the reporter gene phenotype, it is easy to determine whether there is an interaction between the proteins of interest. 

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.