Y2HGold-GAL4 Yeast Two-Hybrid Library Screening Kit（Mating）

Y2HGold-GAL4 Yeast Two-Hybrid Library Screening Kit（Mating）

Principle of the yeast two-hybrid system: Many site-specific transcription activators in eukaryotes generally have two separable domains: the DNA-binding domain (BD) and the transcriptional activation domain (AD). These two domains function independently without affecting each other, but a complete activator that triggers specific gene expression must contain both domains; otherwise, it cannot perform its activation function. When the BD of one activator binds to the AD from another source, it specifically activates the expression of genes bound by the BD. Based on this principle, two proteins of interest can be fused separately to these two domains and co-expressed in the same yeast cell. If the two proteins interact with each other, they will bridge the AD and BD to form a complete transcription activator, thereby activating the corresponding reporter gene expression. By measuring the phenotypes of the reporter gene, it is easy to determine whether there is a molecular interaction between the proteins under investigation. 

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