AH109-pBridge System Yeast Three-Hybrid Interaction Verification Kit

AH109-pBridge System Yeast Three-Hybrid Interaction Verification Kit

The yeast three-hybrid technique is an improvement of the yeast two-hybrid system. This system introduces the pBridge plasmid, which is characterized by its ability to insert two exogenous proteins simultaneously. The protein inserted into MCSI is fused at its N-terminus with the BD binding domain peptide fragment from the Gal4 system, while the protein inserted into MCSII does not fuse with any tag, but has a Pmet25 promoter upstream. This promoter functions only in the absence of methionine (Met), enabling the expression of the downstream protein gene, so experiments must be conducted in methionine-deficient media. 

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.