pUC-pSPYNE173 Vector

pUC-pSPYNE173 Vector

pUC-pSPYNE173 is a prokaryotic bimolecular fluorescence complementation (BiFC) vector modified based on the pUC vector backbone. Its core function is to fuse the target gene with the N-terminal fragment of yellow fluorescent protein (YFP) (SPYNE173, corresponding to amino acids 1-173 of YFP) for expression. When paired with a vector containing the C-terminal fragment of YFP (SPYCE), it allows real-time detection of protein-protein interactions in living cells. It is widely used for preliminary validation of protein interactions in prokaryotic systems (such as E. coli) or lower eukaryotic systems. pUC-pSPYNE173 is an efficient BiFC tool vector for studying prokaryotic protein interactions. Its pUC backbone ensures high-copy amplification, and the SPYNE173 fragment works with a complementary vector to enable fluorescence visualization. It is particularly suitable as an 'initial screening tool' for protein interactions, providing rapid reference for subsequent validation in eukaryotic systems. 

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