Serum Total Iron-Binding Capacity (TIBC) Detection Kit

Serum Total Iron-Binding Capacity (TIBC) Detection Kit

Serum total iron-binding capacity (TIBC) refers to the ability of serum transferrin to bind iron. Its level is closely associated with the occurrence of diseases such as iron-deficiency anemia and acute hepatitis. This kit provides a simple, sensitive, and rapid method for TIBC detection. The principle is that Fe2+reacts with ferrozine to form a fuchsin-purple compound, which has a characteristic absorption peak at 562 nm.Under alkaline conditions, serum transferrin can bind to Fe3⁺, and the remaining unbound Fe3⁺ can be reduced to Fe2+. At this point, the absorbance A₁ is positively correlated with the amount of unbound Fe3+. After acidification, the Fe3+ bound to transferrin is released and further reduced to Fe2+; at this stage, the absorbance A2 is positively correlated with the total amount of Fe3+. The difference between A1and A1 (A2 - A1) is proportional to the TIBC concentration.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.