pML104 Vector

pML104 Vector

The vector belongs to the CRISPR/Cas9 Saccharomyces cerevisiae gene editing system, and its core structure contains the Cas nuclease encoding gene (driven by the GAP promoter) and the single guide RNA (sgRNA) expression cassette (regulated by the SNR52 promoter). By the guide sequence targeting a specific gene into the sgRNA expression cassette, it can guide the Cas9 protein to produce double-strand breaks at the target site in the genome, and then introduce gene mutations through the non-homologous end joining (NHEJ) or homologous recombination (HR) mechanism. It is suitable for point, gene knockout and base editing in Saccharomyces cerevisiae gene function research.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.