Diamine Oxidase (DAO) Assay Kit

Diamine Oxidase (DAO) Assay Kit 

Diamine oxidase (DAO, EC 1.4.3.6) is widely present in animals (intestinal mucosa, lungs, liver, kidneys, etc.), plants, and microorganisms. It catalyzes the oxidation of polyamines to aldehydes, and its activity is closely related to the synthesis of nucleic acids and proteins. It can reflect the integrity and damage degree of the intestinal mechanical barrier.

DAO catalyzes cadaverine to produce aldehydes and hydrogen peroxide (H₂O₂). H₂O₂ oxidizes Fe2⁺ in potassium ferrocyanide to form Fe3⁺. Fe3⁺ further reacts with 4-aminoantipyrine and phenol to generate a red quinone compound, which has a characteristic absorption peak at 505 nm. The activity of DAO is calculated by measuring the absorbance at 505 nm.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.