pKT25 Vector

pKT25 Vector

This vector is one of the core vectors of the bacterial two-hybrid system (e.g., BACTH system), carrying the coding sequence of the N-terminal domain (T25 fragment) of adenylate cyclase. The multiple cloning site is located upstream of the T25 gene, allowing insertion of target gene sequences to construct a fusion protein expression cassette with T25. When co-transformed into E. coli with vectors carrying the T18 fragment (C-terminal domain of adenylate cyclase) (such as pUT18, pUT18C), if the two fusion proteins interact, T25 and T18 can reconstitute active adenylate cyclase, catalyzing cAMP synthesis and activating downstream reporter genes (e.g., lacZ). Protein-protein interactions can be detected through phenotypic changes. It is suitable for prokaryotic protein interaction screening, interaction mechanism analysis, and functional verification.

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2.For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3.The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.