pUT18 Vector

pUT18 Vector

This vector is a basic vector of the bacterial two-hybrid system (e.g., BACTH system), carrying the coding sequence of the C-terminal domain (T18 fragment) of adenylate cyclase. Its multiple cloning site is located differently from pUT18C (usually downstream of the T18 gene), allowing insertion of target genes to construct a C-terminal fusion recombinant protein expression cassette. After co-transformation with vectors carrying the T25 fragment, the interaction of fusion proteins enables T18 and T25 to reconstitute active adenylate cyclase, activating cAMP-dependent reporter gene expression to detect protein-protein interactions. It is suitable for vector construction needs of different fusion modes in prokaryotic protein interaction research.

1.This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2.For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3.The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.