Lacate Dehydrogenase (LDH) Assay Kit

Lacate Dehydrogenase (LDH) Assay Kit 

L-lactate dehydrogenase (L-LDH, EC 1.1.1.27) is widely present in animals, plants, microorganisms, and cultured cells. It is the terminal enzyme in the glycolytic pathway, catalyzing the reversible reaction between pyruvate and L-lactic acid, accompanied by the interconversion between NAD⁺ and NADH.

L-LDH catalyzes NAD⁺ to oxidize L-lactic acid into pyruvate. Pyruvate further reacts with 2,4-dinitrophenylhydrazine to form pyruvate dinitrophenylhydrazone, which turns brown-red in an alkaline solution. The intensity of the color is proportional to the concentration of pyruvate.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.