NAD Kinase (NADK) Assay Kit

NAD Kinase (NADK) Assay Kit 

NAD kinase (NADK, EC 2.7.1.23) is widely present in animals, plants, microorganisms, and cultured cells. It is the only enzyme found in organisms so far that can catalyze the phosphorylation of NAD⁺ to generate NADP⁺. It can catalyze the phosphorylation of NAD(H) using ATP or inorganic polyphosphate [poly(P)] as the phosphoryl donor to produce NADP(H). Therefore, NAD kinase plays an important role in the synthesis of NADP(H) and the regulation of the balance between NAD(H) and NADP(H).

NADK catalyzes the phosphorylation of NAD⁺ to form NADP⁺; NADP⁺ can be reduced to NADPH by glucose-6-phosphate dehydrogenase. Through the hydrogen transfer of PMS (phenazine methosulfate), NADPH reduces oxidized methyl thiazolyl tetrazolium (MTT). The activity of NADK can be reflected by the absorbance value at 570 nm.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.