CoenzymeⅡ NADP(H)Content Assay Kit

CoenzymeⅡ NADP(H)Content Assay Kit 

Coenzyme Ⅱ, NADP(H), is widely present in animals, plants, microorganisms, and cultured cells. The determination of NADP⁺ and NADPH contents allows the calculation of total NADP content (NADPH + NADP⁺) and the NADPH/NADP⁺ ratio. Changes in these parameters are closely related to the pentose phosphate pathway (PPP), biosynthesis, and antioxidant reactions. The NADPH/NADP⁺ ratio is not only one of the main indicators of the cellular redox state but also plays an important regulatory role in the PPP pathway, biosynthesis, and antioxidant metabolism.

NADP⁺ and NADPH in samples are extracted using acidic and alkaline extracts, respectively. Through the hydrogen transfer of PMS (phenazine methosulfate), NADPH reduces oxidized methyl thiazolyl tetrazolium (MTT) to formazan. The absorbance is measured at 570 nm to determine the NADPH content. Glucose-6-phosphate dehydrogenase is used to reduce NADP⁺ to NADPH, thereby determining the NADP⁺ content.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.