Trehalose-6-Phosphate Synthase (TPS) Assay Kit

Trehalose-6-Phosphate Synthase (TPS) Assay Kit

Trehalose is a non-reducing disaccharide composed of two glucose molecules linked by an α,α-1-1 glycosidic bond. It is widely present in various organisms, including bacteria, yeasts, molds, edible fungi, lower plants, insects, invertebrates, and higher plants. The non-reducibility of trehalose determines its stability to acids, alkalis, high temperatures, and other harsh conditions. In addition, it has strong water absorption, which endows it with anti-dehydration effects in organisms. Under stressful conditions, trehalose can protect plants from adverse environmental damage by recognizing external stimuli, generating and transmitting signals, regulating gene expression, and modulating metabolism.

In plants, trehalose is mainly synthesized using glucose as a substrate. This reaction first involves trehalose-6-phosphate synthase (TPS), which catalyzes the reaction between uridine diphosphate glucose (UDPG) and glucose-6-phosphate (G6P) to produce the intermediate product trehalose-6-phosphate. Then, trehalose-6-phosphate phosphatase (TPP) catalyzes the dephosphorylation of trehalose-6-phosphate to generate trehalose. Therefore, determining TPS activity is of great significance for studying plant stress responses.

TPS catalyzes UDPG and G6P to form trehalose-6-phosphate, with the simultaneous production of uridine diphosphate (UDP). UDP, under the action of pyruvate kinase and lactate dehydrogenase, oxidizes nicotinamide adenine dinucleotide (reduced form, NADH) to nicotinamide adenine dinucleotide (oxidized form, NAD⁺). The decrease rate of NADH is proportional to the UDP content, and the TPS activity is reflected by the decrease rate of absorbance at 340 nm.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

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