Pyruvate Kinase (PK) Assay Kit

Pyruvate Kinase (PK) Assay Kit

Pyruvate Kinase (PK, EC 2.7.1.40) is widely present in animals, plants, microorganisms, and cultured cells. It catalyzes the final step in the glycolysis pathway, serving as one of the main rate-limiting enzymes in glycolysis and a key enzyme for ATP production. Therefore, determining PK activity is of great significance.

PK catalyzes the reaction of phosphoenolpyruvate (PEP) and adenosine diphosphate (ADP) to generate adenosine triphosphate (ATP) and pyruvate. Lactate dehydrogenase further catalyzes the reaction of nicotinamide adenine dinucleotide (reduced form, NADH) and pyruvate to produce lactate and nicotinamide adenine dinucleotide (oxidized form, NAD⁺). The activity of PK can be reflected by measuring the decrease rate of NADH at 340 nm.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.