Phosphofructokinase (PFK) Assay Kit

Phosphofructokinase (PFK) Assay Kit

Phosphofructokinase (PFK, EC 2.7.1.11) is widely present in animals, plants, microorganisms, and cultured cells. It is responsible for converting fructose-6-phosphate and ATP into fructose-1,6-bisphosphate and ADP, and is one of the key regulatory enzymes in the glycolytic pathway.

PFK catalyzes fructose-6-phosphate and ATP to generate fructose-1,6-bisphosphate and ADP. Subsequently, pyruvate kinase and lactate dehydrogenase sequentially catalyze the oxidation of nicotinamide adenine dinucleotide (reduced form, NADH) to nicotinamide adenine dinucleotide (oxidized form, NAD⁺). The activity of PFK can be reflected by measuring the decrease rate of NADH at 340 nm.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.