α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit

α-Ketoglutarate Dehydrogenase (α-KGDH) Assay Kit

α-Ketoglutarate dehydrogenase (α-KGDH, EC 1.2.4.2) is widely present in the mitochondria of animals, plants, microorganisms, and cultured cells. It is one of the key regulatory enzymes in the tricarboxylic acid cycle, catalyzing the oxidative decarboxylation of α-ketoglutarate to form succinyl-CoA.

α-KGDH catalyzes α-ketoglutarate, nicotinamide adenine dinucleotide (oxidized form, NAD⁺), and coenzyme A to generate succinyl-CoA, carbon dioxide, and nicotinamide adenine dinucleotide (reduced form, NADH). NADH has a characteristic absorption peak at 340 nm, and the activity of α-KGDH is expressed by the production rate of NADH.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.