pYLCRISPRCas9P35S-B Twin-leaf Plant Single Knockdown Kit

pYLCRISPRCas9P35S-B Twin-leaf Plant Single Knockdown Kit

The pYLCRISPRCas9P35S-B dicotyledon single knockout vector is an efficient and precise gene editing vector developed based on the latest generation of artificial nucleic acid endonuclease CRISPR/Cas9. Compared to traditional knockout techniques such as TALEN and ZFN, it offers simplified operation, higher knockout efficiency, more accurate gene editing, and significantly reduced off-target effects. The pYLCRISPRCas9P35S-B dicotyledon single knockout vector is the first CRISPR/Cas9 system application for single knockout in dicotyledonous plants (with AtU6 promoter), supporting Agrobacterium-mediated random integration and protoplast co-transfection for transient expression systems. The Cas9 gene used in the pYLCRISPRCas9P35S-B vector has been optimized for human codons, and its nuclear localization signal has been demonstrated in literature to function effectively and has been validated across multiple plant species. The vector construction utilizes recombinant integration enzyme fragments and vector assembly methods, with the entire process completed within 1 day, significantly reducing construction time and simplifying operational complexity.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.