pYLCRISPRCas9Pubi-B monocot single knockout kit

pYLCRISPRCas9Pubi-B monocot single knockout kit

The pYLCRISPRCas9Pubi-B monocot single knockout vector is an efficient and precise gene editing vector developed based on the latest generation of artificial nucleic acid endonuclease CRISPR/Cas9. Compared to traditional knockout techniques such as TALEN and ZFN, it offers simplified operation, higher knockout efficiency, more accurate gene editing, and significantly reduced off-target effects. The pYLCRISPRCas9Pubi-B monocot single knockout vector represents the application of the CRISPR/Cas9 system in monocot plants (OsU3 promoter), supporting transient expression systems via Agrobacterium-mediated random integration and protoplast co-transfection. The Cas9 gene utilized in the pYLCRISPRCas9Pubi-B vector has been optimized for human codon reading frames, with its nuclear localization signal having been demonstrated to function effectively in literature reports and validated across multiple plant species. The vector construction employs integrated enzyme recombinant fragments and vector assembly methods, enabling completion within just 1 day, thereby shortening the construction cycle and simplifying the process.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.