pJQ200SK vector

pJQ200SK vector

pJQ200SK was designed as a “suicide vector”, meaning that it cannot stably replicate in a broad range of gramnegative bacteria but can enter host cells. Its core functions are based on two key elements: “SacB gene negative selection”：The SacB gene is lethal to a variety gram-negative bacteria when expressed on a medium containing 5% sucrose. This allows cells that have successfully undergone gene replacement (i.e., lost the pJQ00SK vector) to survive on a sucrose plate, while cells that still retain the vector die, thus facilitating the screening of positive clones. “Conjugative transfer” The vector carries the “oriT”, which allows mobilization from one bacterium (e.g., a donor bacterium) to another recipient bium through conjugation.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.