R5421 Chemically Competent Cell

R5421 Chemically Competent Cell

The R5421 brewing yeast strain is a K/potassium ion-deficient strain, also known as CY162 in the literature, and is of MATα type. It is commonly used in assays for identifying K/potassium ion transport proteins, and can also be used in assays for identifying K/potassium ion channels or sodium-potassium ion pumps. The transformation markers are: ura3, leu2. This strain can grow normally in media containing 100mM KCl, grows slowly in media containing 5-10mM KCl, and ceases to grow when the KCl concentration in the medium is below 0.5mM. R5421 (CY162) cells stop growing under these conditions. R5421 competent cells are prepared using a special process and can be stored at -80°C for 12 months. The pGADT7 plasmid (7988bp, Amp^R) shows a transformation efficiency of >10⁴ cfu/μg DNA.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.