X33

X33

X33 is a Pichia pastoris strain, belonging to eukaryotic cells. General antibiotics for prokaryotes, such as kana and ampicillin, are ineffective for yeast, so in order to prevent prokaryotes such as Escherichia coli from polluting yeast culture strains, some antibiotics such as ampicillin and kanamycin are often added to the culture medium to inhibit the pollution and growth of bacteria. The suitable growth temperature of Pichia pastoris is 28-30℃, and the temperature over 32℃ is harmful to protein expression and may lead to cell death. X33 Pichia pastoris can grow in YPG medium. X33 Pichia pastoris is recommended to express recombinant plasmid containing Zeocin resistance, such as pPICZ A,B,C B and C series vectors. When screening X33 recombinant transformed strain, the working concentration of Zeocin antibiotic was 100ug/ml. X33 Pichia pastoris contains AOX1 gene, and the transformed strain produced is Mut+ genotype.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.