KM71

KM71

KM71 is a Pichia pastoris strain, belonging to eukaryotic cells. General antibiotics for prokaryotes, such as kana and ampicillin, are ineffective for yeast, so in order to prevent prokaryotes such as Escherichia coli from polluting yeast culture strains, some antibiotics such as ampicillin and kanamycin are often added to the culture medium to inhibit the pollution and growth of bacteria. The phenotype of KM71 Pichia pastoris is MutS, so KM71 transformant can only produce His+MutS strain. The suitable growth temperature of Pichia pastoris is 28-30℃, which can generally be set at 30℃. The temperature exceeding 32℃ is harmful to protein expression and may lead to cell death. Pichia pastoris KM71 is histidine deficient (His4 genotype). If the expression vector carries histidine gene, it can compensate the histidine deficiency of the host strain, so transformants can be screened on the medium without histidine. The probability of spontaneous mutation of these recipient bacteria to histidine wild type is generally lower than 10 -8. KM71 Pichia pastoris can grow in YPD medium or in minimal media supplemented with histidine, but it cannot grow in minimal media alone.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.