GV3101(PSoup-P19)

GV3101(PSoup-P19)

P19 protein, which is derived from tomato witches' broom virus, can inhibit the RNA silencing effect of host on foreign genes, improve the stability of heterologous gene transcripts, and then promote the expression of heterologous proteins, and is widely used in transient expression systems of transgenic plants, tobacco leaves, Arabidopsis leaves, tomato leaves or protoplasts. GV3101 strain is a C58 type background, and its nuclear gene contains a screening label-rifampicin resistance gene rif. In order to facilitate the transformation operation, this strain carries a carmine-type Ti plasmid pMP90 (pTiC58DT-DNA) without its own transport function, and this plasmid contains vir gene (vir gene is T-DNA inserted into plants.The essential element of genome, the T-DNA transfer function of pMP90 (pTiC58DT-DNA) plasmid itself is destroyed, but it can help the transferred binary vector T-DNA transfer smoothly. PMP90 (pTiC58DT-DNA) Ti plasmid contains screening label: gent, which endows GV3101 strain with gentamicin resistance; Transfer help plasmid into GV3101 strain: pSoup-p19 is GV3101(pSoup-p19) strain, which can help the replication of plasmids such as pGreen, 62SK and pGs2 in Agrobacterium, and endow the strain with tetracycline (tet) resistance. It is suitable for transgenic operation of Arabidopsis thaliana, tobacco, corn, potato and other plants.

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