ER2566

ER2566

ER2566 strain is a prokaryotic strain with super high transformation efficiency, which is developed by NEB company and originated from BL21. Lac promoter initiates the expression of downstream T7RNA polymerase, which can be used for high-level protein expression of T7 promoter expression vectors (such as pET series). FhuA2 endows ER2566 strain with resistance to phage T1. At the same time, ER2566 is a strain deficient in lon and ompT protease. The deletion of Lon protease and extracellular protease OMPT can effectively inhibit the degradation of the expressed heterologous protein in Escherichia coli. The existence of δ (MCRC-MRR) 114 and mcr-73 mutations makes it impossible for ER2566 strain to label and restrict foreign DNA, which improves the transformation efficiency of foreign methylated DNA.

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3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.