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life sciences

SM10λpir

Article number:
SP8253
Specifications:
300μl
Price:
¥680.00
Pave:
-20℃ (short term) /-80℃ (long term)
  • Overview
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SM10λpir

English name

SM10λpir Escherichia coli strain

Chinese name

SM10λpir大肠杆菌菌株

Specification

300μl

Sample storage

-20℃ (short term) /-80℃ (long term)

Ransportation guide

Ice pack shipping

SM10λpir is an Escherichia coli engineering strain specially designed for indirect bacterial transfer. Its core feature is that the chromosome is integrated with the junction transfer (tra) gene of RP4 plasmid, which can autonomously start the transfer of DNA to other strains. The strain is recombinant defective (recA), which ensures the stability of foreign DNA. It carries λpir elements, allowing suicide vectors (such as pDM4) that rely on π protein replication to exist stably. SM10λpir is suitable for transferring plasmids from Escherichia coli to Gram-negative bacteria which are difficult to be directly transformed, such as Agrobacterium, and is a common tool for constructing gene knockout mutants.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.

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