Filamentous fungi gene editing in

Service Introduction

Fungal filamentous fungi are multicellular microorganisms of critical importance in industrial, agricultural, and pharmaceutical applications. Punter Biotech employs third-generation editing technologies represented by the CRISPR/Cas9 system, which enables precise genome modification in filamentous fungi with high efficiency and simplicity. These techniques include gene knockout, insertion of exogenous sequences, and introduction of point mutations, facilitating strain functional optimization and metabolic pathway reconstruction.

Service Advantages

▶Extensive Experience: Possesses extensive expertise in filamentous fungal gene editing, with a seasoned team capable of efficiently constructing filamentous fungal mutants;

▶Quality Assurance: All gene-editing sites are rigorously sequenced and validated to ensure product quality.

Service Process

Article Case

The role of the Flb protein family in the life cycle of Aspergillus niger

academic journal：Antonie van Leeuwenhoek

Growth of A. niger MA234.1 and the ∆flbA, ∆flbB, ∆flbC, ∆flbD and ∆flbE strains on PDA and on MMA with different carbon sources (A), as well as biomass (B), number of spores (C), and number of spores per mg mycelium (D) of cultures grown on MMA with glucose (MMA-G). Cultures were grown for 5 (A) and 7 (B) days from a point inoculum or grown for 3 days after confluent spreading of spores on a plate(C, D). To determine biomass, colonies were grown in between two perforated polycarbonate membranes, while spores were harvested from colonies that had grown on MMA-G in the absence of the membranes. Statistical analysis was done with One-way ANOVA with different letters indicating statistical differences.

Applicable Fields

▶Enhancing industrial enzyme and chemical yields: By modifying industrial fungal chassis (e.g., Aspergillus niger and Aspergillus grayansporus) used for producing enzyme preparations and organic acids, the metabolic networks can be optimized to significantly increase the yield of target products.

▶ Development of novel drugs: Activating or knocking out silent gene clusters (BGCs) in fungi to discover and efficiently produce novel natural products with antibacterial, anticancer, and other therapeutic activities.

▶Enhanced cultivation of edible fungi and crop disease control: Genetic modification of shiitake mushrooms improved biomass conversion efficiency, while genetic editing of Ganoderma lucidum doubled the yield of triterpenoid medicinal components. Additionally, knockout of pathogenic genes in rice blast fungus and Fusarium oxysporum provided novel targets for developing green control strategies.

▶Gene function elucidation: Construct gene knockout or mutant strains, and combine multi-omics analyses to comprehensively elucidate gene regulatory networks and metabolic pathways.