Yarrowia lipolytica gene editing of

Service Introduction

Yarrowia lipolytica is a highly promising industrial microbial chassis, renowned for its unique lipid metabolism capabilities and robust genetic plasticity. Punter Biotech employs third-generation editing technologies represented by the CRISPR/Cas9 system, which enables precise genome modifications in Yarrowia lipolytica with high efficiency and simplicity—including gene knockout, foreign sequence insertion, and point mutation introduction—to achieve strain functional optimization and metabolic pathway reprogramming.

Service Advantages

▶Extensive Experience: We have accumulated extensive expertise in the field of gene editing for lipolytic Saccharomyces cerevisiae. Our experienced team can assist you in rapidly constructing mutant strains of lipolytic S. cerevisiae.

▶ Quality Assurance: All gene-editing sites are rigorously sequenced and validated to ensure product quality.

Service Process

Article Case

Optimizing the CRISPR/Cas9 system for gene editing in Yarrowia lipolytica

academic journal：Engineering Microbiology

Optimization of the CRISPR/Cas9 system for improved gene disruption efficiency.(A)Schematic of different promoters of sgRNA used in this study.sgRNA is a single guide RNA,HH is the hammerhead ribozyme,and HDV is the hepatitis delta virus ribozyme.Synthetic RNA polymerase (Pol)III promoters were generated by inserting the SNR52p or SCR1p sequences immediately upstream of tRNAGly.Polyt,which is a string of four thymines,served as the Pol III terminator. (B)URA3 disruption efficiencies achieved with different sgRNA promoters in the PO1f-Cas9-URA3 strain.(C)Validation of the efficiency of URA3 gene disruption. Transformants carrying different sgRNA promoters were randomly selected and streaked onto YPD and SC-URA plates,respectively.In total,20 colonies from each biological parallel were picked and tested to calculate the gene disruption efficiency of the system.(D)URA3 disruption efficiency under different Cas9 expression conditions.

Applicable Fields

▶Metabolic Engineering and Chemical Production: By knocking out competitive pathways and overexpressing key enzymes, fatty acid esters, medium-chain fatty acids, and microbial oils for aviation fuel can be efficiently produced.

▶Functional Genomics and Cell Factory Optimization: Systematic knockout of core metabolic genes facilitates the elucidation of complex metabolic mechanisms, such as lipid accumulation, at the molecular level.

▶Production and Modification of Proteins/Enzymes: Gene editing offers an effective solution to industry challenges such as low yield and susceptibility to degradation of recombinant proteins.

▶ Industrial chassis strain construction: By removing bacterial vector elements and antibiotic resistance genes, while introducing multiple nutritional deficiency markers, a new generation of chassis strains compliant with industrial safety and biosecurity standards can be developed.