pML105 Vector

pML105 Vector 

The pML105 vector is based on the pML104 scaffold but replaces the Ura selection marker with Trp, belonging to the CRISPR/Cas9 gene-editing system for Saccharomyces cerevisiae. Its core structure comprises the Cas9 nuclease encoding gene (driven by a GAP promoter) and a single guide RNA (sgRNA) expression cassette (regulated by a SNR52 promoter). By inserting a guide sequence targeting specific genes into the sgRNA expression cassette, Cas9 can be directed to induce double-strand breaks at designated sites in the S. cerevisiae genome, thereby introducing genetic mutations via non-homologous end joining (NHEJ) or homologous recombination (HR). This vector is suitable for site-specific mutation, gene knockout, and base editing in functional studies of S. cerevisiae genes.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.