pGK415-rtetTAK8N, L131L Vector

pGK415-rtetTAK8N, L131L Vector 

The plasmid pGK415-rtetTAK8N, L131L was constructed by the team of Jun Ishii at Kobe University in Japan and published on Addgene (plasmid number #165969). It is a plasmid designed for gene-induced expression in yeast. The expressed fusion protein (rtTA) does not normally bind to DNA. Upon addition of doxycycline (Dox) to the culture medium, rtTA undergoes conformational changes, binds to the promoter containing the tetO operon sequence, and activates downstream gene transcription via its VP16 domain. Consequently, this vector is widely utilized to construct "drug-on" inducible expression systems for investigating the function or toxicity of specific genes.

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2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.