po1f Chemically Competent Cell

po1f Chemically Competent Cell

The Yarrowia lipolytica ATCC MYA-2613 strain is an engineered derivative of the wild-type strain W29 (ATCC 20460). Due to its well-characterized genetic background and multiple advantageous traits, po1f has become one of the most widely used Yarrowia lipolytica host strains in synthetic biology, metabolic engineering, and industrial fermentation applications. The xpr2-322 and axp2-deltaNU49 mutations in this strain prevent the production of major extracellular proteases, conferring a significant advantage when expressing recombinant proteins sensitive to proteolytic degradation. The po1f strain is a dual nutritional deficiency strain for uracil and leucine; this characteristic makes it an ideal host for plasmids carrying URA3 or LEU2 selection markers or for gene knockout constructs, facilitating genetic manipulation and transformant screening. po1f is a strictly aerobic yeast that requires an ample supply of oxygen for growth, with a doubling time of approximately 1.5–2 hours. Due to its robust lipid metabolism capacity and high efficiency in heterologous protein expression, po1f is extensively utilized in the fields of biofuels, food additives, pharmaceuticals, and environmental protection: 1) Heterologous protein expression: for the production of industrial enzymes and therapeutic proteins; 2) Metabolic engineering and synthetic biology: as a chassis strain for producing various high-value chemicals, such as erythritol, α-farnesene, β-carotene, and β-violetone; 3) Lipid production: as an oil-producing yeast for the production of neutral lipids and specialty lipids. The pINA1269, pYL1269GH, and pYL1269FH plasmids are protein expression vectors for the po1f gene of Yeast saccharomyces; the screening marker is LEU, and these plasmids can be screened using SD-LEU agar plates. Yeast saccharomyces po1f competent cells are prepared using a specialized protocol and can be stored at –80°C for up to 12 months. The pINA1269 plasmid (7298 bp, AmpR) exhibits a transformation efficiency>10³ cfu/μg DNA.

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