Bacterial Single Hybrid Interaction Verification Kit

Bacterial Single Hybrid Interaction Verification Kit

Our company has developed a bacterial single-hybrid validation kit that leverages the in vivo environment of Escherichia coli as a tool for studying DNA–protein interactions and for identifying inhibitors targeting specific transcription factor–DNA interactions. The core principle involves placing the DNA sequence under investigation (the bait) upstream of a reporter gene; when it specifically binds to a co-expressed transcription factor (the prey), it activates the expression of the His3 and aadA reporters (streptomycin resistance, which is not commonly used), thereby allowing the interaction to be determined through a bacterial survival assay. In this protocol, the optimal concentration of 3-AT for detecting the bait Escherichia coli strain and the validation of interactions were both performed using the dilution-point plate method. Compared with the spread-plating method, the point-plating method provides a more intuitive visualization of DNA–protein interactions and reduces the amount of culture medium and 3-AT required. This protocol also includes an analysis of common bacterial single-hybrid results.

1. This product is for scientific research use only. Please do not use it for medical, clinical diagnosis or treatment, food and cosmetics, etc. Please not store it in a general residential area.

2. For your safety and health, please wear a lab coat and disposable gloves and masks when operating.

3. The experimental can be affected by a variety of factors, and the relevant treatment is limited to the product itself, and does not involve other compensation.